RESUMEN
Background: COVID-19, the highly contagious respiratory disease, has become a major threat to humanity, and its extrapulmonary effects were also evident. Heart failure (HF) may be the result of myocardial damage associated with COVID-19. Methods: : To understand the relationship between SARS-COV-2 and HF, we used bioinformatics analysis to identify common pathways and molecular biomarkers for HF and COVID-19. In this study, two datasets (GSE152418, GSE57338) from Gene Expression Omnibus (GEO) were used to identify differentially expressed genes (DEGs) of SARS-COV-2 infection in HF patients to find common pathways and drug candidates. Results: : A total of 123 common DEGs were identified in the two datasets. Using a variety of bioinformatics tools, we first constructed protein-protein interactions (PPI) and then identified hub genes that could be served as potential biomarkers or novel therapeutic strategies. In addition, some common associations between HF and the progression of COVID-19 infection were found by using functional under ontological terms and pathway analysis. Through the datasets, we also identified transcription factor-gene interactions, protein-drug interactions, and co-regulatory network of DEGs-miRNAs with common DEGs. We built gene-disease association network to represent diseases associated with mutual DEGs. Conclusions: : Our study has identified the candidate hub genes and drugs that might become a new therapeutic target for novel coronavirus vaccine development and treatment in COVID-19 and HF.
Asunto(s)
COVID-19 , Insuficiencia CardíacaRESUMEN
OBJECTIVE: To explore the clinical characteristics of nine cases of clustered Chlamydia psittaci pneumonia under the COVID-19 pandemic. METHODS: Total of nine cases diagnosed with C. psittaci pneumonia during Dec 2020 were enrolled in the study, of whom eight cases were treated in the Department of Miyun District of Peking University First Hospital and one case were from TCM Hospital of Beijing Miyun Traditional Chinese Medicine Hospital. The clinical data of the patients were retrospectively analyzed, and the clinical characteristics of the disease and treatment were summarized. RESULTS: The onset time of nine patients was concentrated, and all of them had activities or living history in pigeon breeding area. All patients had different degrees of fever, 77.80% of whom were high fever, accompanied by cough and expectoration. The white blood cell count was normal and the neutrophils were increased. C-reactive protein level in 8 cases was increased and D-dimer was elevated in six cases. Chest CT scanning showed high-density consolidation in unilateral lungs, with bronchial inflatable signs, especially in lower lungs, but bilateral lung changes were not common. One death was reported as multiple variations in ground-glass opacity of bilateral lungs. Eight patients were treated and recovered with no observable inflammatory lesions and esidual fibrosis. Two patients' specimens were detected with C. psittaci nucleic acid by metagenomics next-generation sequencing(mNGS) technology. Seven patients underwent serum antibody test for C. psittaci, all of which were positive. Six patients were under respiratory tract pathogenic testing, all of which were the combined infection of Klebsiella. Quinolone antibiotics were used in the treatment of eight patients, either alone or in combination with other drugs. Two patients were treated with tetracyclines. Eight patients were cured, and one died clinically. CONCLUSION: Patients should be inquired their contact history in detail when encountering an unexpected clustered outbreak of pneumonia. The mNGS is efficient in identifying the pathogenic bacteria, which can help to reduce the burden of disease ultimately. Except for tetracyclines, quinolones have shown good therapeutic effects in the treatment of C. psittaci pneumonia.